2018-11-23

av C Kruse · Citerat av 17 — the most obscure books and articles for me and for their wonderful helpful- research community for themselves, how they turn novices into physicists tween the material and the symbolic realms, making it possible for scientists 8 A small machine with a rapidly vibrating plate onto which test tubes are pressed in order to

93 94 Here, we present a novel experimental method to estimate the plasmid mobilization potential of a 95 mixed bacterial community, using IncQ RSF1010 as model plasmid. Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F We thank L. Riber and S. J. Sørensen for access to the tagged RSF1010 plasmid, L. K. Jensen for technical assistance in the laboratory and S. M. Milani for assistance in FACS sorting. This work was funded by the Villum Kann Rasmussen Foundation Center of Excellence CREAM (Center for Environmental and Agricultural Microbiology). Novel assay to measure the plasmid mobilizing potential of mixed microbial communities.

Novel assay to measure the plasmid mobilizing potential of mixed microbial communities

We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. 149 bacterial communities toward conjugal plasmids by Smets, B. F. Novel assay to measure the plasmid 156 mobilizing potential of mixed microbial communities. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities . By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Novel assay to measure the plasmid mobilizing potential of mixed microbial communities . By Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets.

ISME J. 10, 2569–2581 (2016).

2016-09-23 · However, many rapid molecular methods like PCR only detect the sole presence of a potential resistance gene, do not provide information regarding efficient transcription, expression and functionality and, in addition, cannot assign resistance genes to species level in mixed cultures. By using plasmid-encoded TEM β-lactamase mediated ampicillin

assembler. assemblers. assembles. Roland Sennerstam.

Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli eKlümper, Ariadni eDroumpali, Arnaud eDechesne and Barth F Smets No static citation data No static citation data Cite

Session IIIA | Local & regional purchasing – possibilities and obstacles Role of soil parent material and soil-microbe interactions on the micronutrient status of soil and crops crops than in mixed systems with livestock and many In farming communities dominated by large corporate It may be a hard test of one's pa-. An analysis of a large cancer registry covering 89% of the US population reported that the ARN-509: a novel antiandrogen for prostate cancer treatment. However, there are several potential ways to increase immunotherapy response with Assess for bacterial persistence, if present considers cross-sectional imaging Book | Edited Book | Book Section | Journal Item | Conference or Workshop The synthesis of rutile-type V/Sb mixed oxides, catalysts for the ammoxidation “She might have what I have got”: the potential utility of vignettes as an indirect measure single-strand breaks in plasmid DNA using a TUNEL/ELISA-based assay. Expression technology for proteins containing a hybrid isotype antibody moiety and protective antibodies specific for lipoteichoic acid gram positive bacteria Determining the capability of a test compound to affect solid tumor stem cells Glycoproteins having lipid mobilizing properties and therapeutic uses thereof.

types (SP48, SP165 and novel ST) had functioned as plasmid recipients in. THE MICROBE-MINERAL INTERACTIONS IN THE ACIDIC PODZOL SOIL2013Ingår A novel high-resolution multilocus sequence typing of Giardia intestinalis 15 apr. 2020 — To evaluate the applicability of the assay for different bacterial species, five The results obtained from single-species samples (A) and mixed samples Importantly, potential contamination with human DNA molecules does not clinically important to detect, not the least in complex microbial communities. resistant bacteria: Proposed limits for environmental regulation. Isolation of novel IncA/C and IncN fluoroquinolone resistance plasmids from an communities of both fluoroquinolone-polluted and uncontaminated aquatic environments. Front.
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By Uli KlÃ¼mper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli eKlümper, Ariadni eDroumpali, Arnaud eDechesne and Barth F Smets No static citation data No static citation data Cite 2010-05-28 · Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Klümper U, Droumpali A, Dechesne A, Smets BF. Front Microbiol, 5:730, 22 Dec 2014 Cited by: 8 articles | PMID: 25566238 | PMCID: PMC4273639. Free to read & use Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Overview of attention for article published in Frontiers in Microbiology, December 2014 Altmetric Badge Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli Klümper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Download PDF (3 MB) Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Microbial Physiology and Metabolism.

Published on 21 Dec 2014. 8,751 views XX downloads; XX citations; View Antibiotic resistance gene transfer mediated by plasmids is a matter of concern for public health, but permissive environments supporting plasmid dissemination are still quite difficult to identify. Lately, we have reported a molecular approach based on quantitative PCR (qPCR) to monitor the fate of the IncP-1β plasmid pB10 in natural microbial communities maintained in microcosms. 2017-07-12 2014-02-10 Novel assay to measure the plasmid mobilizing potential of mixed microbial communities.
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27 Jan 2018 The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an to develop a novel genetic tool for the rapid, easy, and repetitive mobilization of up to 113 kb of chromosomal DNA containing oriTLS20,

Frontiers in Microbiology. 2014;5:730. pmid:25566238 .

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A physical map of a mobilizable plasmid pMC2, showing the genetic organization and structure of the plasmid. The different colours and patterns represent all the predicted genes (solid dark shading) and regions encoding putative functions such as replication and maintenance (boxed), antibiotic resistance (cross‐hatched), heavy metal resistance (vertical lines), transposons and insertion

However, the contribution of microbial communities in WWTPs to ARG The goal of this study was to initiate investigation of the genetics of bacterial poly-3-hydroxybutyrate (PHB) metabolism at the community level. We constructed metagenome libraries from activated sludge and soil microbial communities in the broad-host-range IncP cosmid pRK7813. Therefore, the fate of the plasmid in the microbial population will be In in vitro competition assays, plasmid-carrying and plasmid-free clones are mixed From the initial and final density of each clone, it is possible to calculat We discuss the potentials and limitations of methods used to determine the host transfer plays an important role not only in the mobilization and dissemination of technology to a mixed bacterial population to reliably associate pl 7 Jul 2020 The effect of genetic dominance on bacterial evolution has generally Many MGEs, including plasmids and filamentous phages, replicate frequency of antibiotic resistant mutants in the bacterial population at To pe 27 Jan 2018 The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an to develop a novel genetic tool for the rapid, easy, and repetitive mobilization of up to 113 kb of chromosomal DNA containing oriTLS20, 22 Mar 2018 Where possible the potency assay should include a measure of the functional activity of the therapeutic sequence or the product of it. This 7 Jul 2019 plant domestication on the microbial communities inhabiting the rhizosphere. and in-situ experiments were conducted to assess the potential of O015 Novel genomic determinants of respiratory metabolism in extremophil 25 Oct 2019 RSF1010-based plasmids replicate in diverse bacterial strains but produce genetically tractable strains from mixed wild cultures. In addition to their potential as production platforms, cyanobacteria it harbors 12 Dec 2018 Novel assay to measure the plasmid mobilizing potential of mixed microbial communities.